I've never seen a colony that large except when I spread one out doing some landscaping, but they built a new centralized colony within a week. Is it possible the ants are foraging over the entire pen? When foraging they will sometimes excavate to some extent near a food source like spilled feed or grain. The boiling water is only effective on the main colony. It won't kill it but they will usually move on a few or few hundred feet and do it all over again. How wet is the area around the chicken pen? If the ground is saturated and assuming your pen is elevated then they don't have anywhere else to go and you may hve to use some form of insecticide to rid your pen of them.
They don't seem to bother the chickens too bad except for the chicks and hens on a nest. If I have a broken egg in a nest box I change the bedding and clean it out the best I can. I try to limit spilled feed in the pen. I use diatomaceous earth in the covered portion of my pen, and elsewhere during dry weather, when I have trouble with ants or any other insects. It doesn't kill them all outright but does seem to work given a little time.
They don't seem to bother the chickens too bad except for the chicks and hens on a nest. If I have a broken egg in a nest box I change the bedding and clean it out the best I can. I try to limit spilled feed in the pen. I use diatomaceous earth in the covered portion of my pen, and elsewhere during dry weather, when I have trouble with ants or any other insects. It doesn't kill them all outright but does seem to work given a little time.