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I really think the reason I can tell our roo from the pullets even at two weeks is the yellow dye in his fuzz from the hatchery ....but it is twice the size of the rest of the chicks and slightly pink. we try to handle them daily and examine and hold each breifly passing them between two adults...

I am not sure bout all breeds, I have black australorps and the marked male i purchased has a comb twice as big as any of the pullets at 2 weeks